ABSTRACT
A paracoccidioidomicose (Pbmicose) é uma doença granulomatosa de comprometimento sistêmico causada pelo fungo dimórfico Paracoccidioides brasiliensis. As manifestações clínicas são diversas e têm relação com a resposta imune do hospedeiro. O diagnóstico de Pbmicose tem evoluído nos últimos anos devido o advento das provas sorológicas. Estas são usadas não só para diagnósticos como também para determinar a eficácia terapêutica antifúngica durante e após o tratamento. O objetivo do estudo foi correlacionar os resultados dos testes sorológicos por imunodifusão dos pacientes com pbmicose com as formas clínicas da doença na população de São José do Rio Preto. Para tanto foram utilizadas informações de 79 prontuários de pacientes atendidos no Hospital de Base da Faculdade de Medicina de São José do Rio Preto, no período de2000 a 2003. O resultado obtido mostra prevalência para população masculina, com idade entre 35 e 55 anos, sendo a maioria trabalhadora ou moradora da zona rural. Os pulmões, a mucosa oral e gânglios foram as áreas anatômicas mais comprometidas. O método diagnóstico mais utilizado foi a biópsia de lesão (70,4%), seguido de imagens radiológicas, compatíveis em 49% dos casos, e prova sorológica por imunodifusão radial positiva em 29 pacientes (40,8%). O tratamento de escolha foi a sulfadiazina, seguida pelo uso de sulfametoxazol +trimetoprim. Foi possível correlacionar os títulos de anticorpos com a forma clínica apresentada, tendo em vista a diminuição destes frente ao sucesso terapêutico.
Paracoccidioidomycosis (Pbmicosis) is a granulomatous disease with systemic impairment caused by dimorphic fungi Paracoccidioides brasiliensis. The clinical manifestations are diverse and have relation with the immune answer of the host. Paracoccidiodomycosis diagnosis has evolved in these last years due to the advent of the serologic tests. These are used not only for diagnosis, but also to determine the antifungical therapeutic effectiveness during and after the treatment. The objective of this study was to correlate the results of immunodiffusion serologic tests of patients with Paracoccidioidomycosis with the clinical forms of the disease in the population of São José do Rio Preto. Seventy nine medical records of patients assisted in the Hospital de Base of Medical School , São José do Rio Preto from 2000 to 2003 were used. The results showed that male population aged between 35 to 55 was the most significant ; the majority was workers or inhabitants from rural area. Lungs, oral mucosa and lymph nodes were the structures more frequently impaired.The biopsy of injury was the diagnostic method most used (70.4%), the radiological images were compatiblein 49% of the cases and serologic tests had been positive in 29 patients (40.8%). The chosen treatment was the sulfadiazine, followed by the administration of sulfametaxazol + trimetoprim. It was possible to correlate antibodies titers with the clinic tests; consequently the reduction of these regarding the therapeutical success.
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Clinical Laboratory Techniques , Clinical Diagnosis/statistics & numerical data , Paracoccidioidomycosis/diagnosisABSTRACT
One hundred sixty eight bacterial strains, isolated from soil and samples of vegetable in decomposition, were screened for the use of citrus pectin as the sole carbon source. 102 were positive for pectinase depolymerization in assay plates as evidenced by clear hydrolization halos. Among them, 30(per cent) presented considerable pectinolytic activity. The cultivation of these strains by submerged and semi-solid fermentation for polygalacturose production indicated that five strains of "Bacillus" sp. produced high quantities of the enzyme. The physico-chemical characteristics, such as optimum pH of 6.0-7.0, optimum temperatures between 45ºC and 55ºC, stability at temperatures above 40ºC and in neutral and alkaline pH, were determined